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Rabbit Anti-Nestin/PE-Cy5.5 Conjugated antibody (bs-0006R-PE-Cy5.5)
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說(shuō) 明 書: 100ul  
100ul/2980.00元
大包裝/詢價(jià)
產(chǎn)品編號(hào) bs-0006R-PE-Cy5.5
英文名稱1 Rabbit Anti-Nestin/PE-Cy5.5 Conjugated antibody
中文名稱 PE-Cy5.5標(biāo)記的巢蛋白/神經(jīng)上皮干細(xì)胞蛋白抗體
別    名 ESTM 46; FLJ 21841; FLJ21841; Intermediate filament protein; Nbla00170; Nes; NEST_HUMAN; Nestin.  
規(guī)格價(jià)格 100ul/2980元 購(gòu)買        大包裝/詢價(jià)
說(shuō) 明 書 100ul  
研究領(lǐng)域 腫瘤  細(xì)胞生物  發(fā)育生物學(xué)  神經(jīng)生物學(xué)  干細(xì)胞  腫瘤細(xì)胞生物標(biāo)志物  
抗體來(lái)源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Human, 
產(chǎn)品應(yīng)用 IF=1:50-200 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 178kDa
性    狀 Lyophilized or Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Nestin
亞    型 IgG
純化方法 affinity purified by Protein A
儲(chǔ) 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
產(chǎn)品介紹 background:
There are a certain number of neural stem cells, which can be potentially differentiated into the neurons or neuroglia, in the adult mammalian brain. These neural precursors are located in the subependymal cell layer of the ventricle. Those precursors isolated from embryonic brain can be divided and differentiated into the neurons and glia in vitro and this is regulated by a series of growth-factor actions.

Function:
Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells (By similarity).

Subunit:
Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, tis protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3 (By similarity).

Tissue Specificity:
CNS stem cells.

Post-translational modifications:
Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.

Similarity:
Belongs to the intermediate filament family.

Database links:

Entrez Gene: 10763 Human

Entrez Gene: 18008 Mouse

Entrez Gene: 25491 Rat

Omim: 600915 Human

SwissProt: P48681 Human

SwissProt: Q6P5H2 Mouse

SwissProt: P21263 Rat

Unigene: 527971 Human

Unigene: 331129 Mouse

Unigene: 9701 Rat




Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.


結(jié)構(gòu)蛋白(Structural Proteins)
Neural Stem Cell Marker (神經(jīng)干細(xì)胞標(biāo)志物)

在動(dòng)物體早期發(fā)育過(guò)程中,中樞神經(jīng)系統(tǒng)發(fā)育尤為重要 。Nestin是一種中等纖維蛋白,它在哺乳動(dòng)物神經(jīng)前體細(xì)胞中表達(dá)較高,目前已被廣泛用于神經(jīng)前體細(xì)胞的重要標(biāo)志分子。研究Nestin的表達(dá)調(diào)控規(guī)律,將有助于人們認(rèn)識(shí)神經(jīng)前體細(xì)胞的生物特性。此抗體還表達(dá)中樞神經(jīng)系統(tǒng)干細(xì)胞及神經(jīng)上皮細(xì)胞。
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