| 產品編號 | bs-20416R |
| 英文名稱 | Rabbit Anti-LC3C antibody |
| 中文名稱 | 微管相關蛋白輕鏈3C抗體 |
| 別 名 | LC3 like protein 2; MAP1 light chain 3 like protein 3; Microtubule-associated proteins 1A/1B light chain 3C; Autophagy-related protein LC3 C; Autophagy-related ubiquitin-like modifier LC3 C; MAP1 light chain 3-like protein 3; MAP1A/MAP1B light chain 3 C; MAP1A/MAP1B LC3 C; Microtubule-associated protein 1 light chain 3 gamma; MAP1LC3C; MLP3C_HUMAN. |
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Specific References (1) | bs-20416R has been referenced in 1 publications.
[IF=0] Haider F. Ghazi et al. Immunohistochemical Expression of Xenophagy Proteins in Helicobacter pylori and None Helicobacter pylori Gastritis. J Pure Appl Microbiol,2018 12(4), 1795-1800 Dec. 2018 IHC ; Human.
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| 研究領域 | 心血管 信號轉導 細胞骨架 細胞自噬 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human (predicted: Mouse) |
| 產品應用 | Flow-Cyt=1μg/Test,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 16kDa |
| 細胞定位 | 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human LC3C: 1-50/147 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | Preservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
A major contributor to cellular homeostasis is the ability of the cell to strike a balance between the formation and degradation/removal of its cellular components. This process of internal cellular turn-over is called autophagy (self-eating), and is facilitated by a pathway of around 16 interacting proteins in the human. LC3, a ubiquitin-like modifier protein, is the human homolog of yeast Apg8 and is involved in the formation of autophagosomal vacuoles, called autophagosomes. There are three isoforms of human LC3 (named MAP1LC3A, MAP1LC3B, and MAP1LC3C), which exhibit different tissue distributions. A disruption to the autophagic process is now associated with the progression of several cancers, neurodegenerative disorders and cardiac pathologies, where LC3 is widely employed as a marker for autophagy. Function: Probably involved in formation of autophagosomal vacuoles (autophagosomes). Subunit: 3 different light chains, LC1, LC2 and LC3, can associate with MAP1A and MAP1B proteins. Subcellular Location: Cytoplasm, cytoskeleton. Endomembrane system; Lipid-anchor. Cytoplasmic vesicle, autophagosome membrane; Lipid-anchor. Tissue Specificity: Most abundant in placenta, lung and ovary. Similarity: Belongs to the MAP1 LC3 family. SWISS: Q9BXW4 Gene ID: 440738 Database links: Entrez Gene: 440738 Human Omim: 609605 Human SwissProt: Q9BXW4 Human Unigene: 534971 Human |
| 產品圖片 |
Blank control:293T.
Primary Antibody (green line): Rabbit Anti-LC3C antibody (bs-20416R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
