| 產品編號 | bs-9924R |
| 英文名稱 | Rabbit Anti-WDFY3 antibody |
| 中文名稱 | WDFY3蛋白抗體 |
| 別 名 | Alfy; Autophagy linked FYVE protein; KIAA0993; MGC16461; WD repeat and FYVE domain containing 3; ZFYVE25; WDFY3_HUMAN. |
| 研究領域 | 心血管 細胞自噬 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse,Rat (predicted: Human,Rabbit,Pig,Sheep,Cow,Dog,Horse) |
| 產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:50-200,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 395kDa |
| 細胞定位 | 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human WDFY3: 951-1050/3526 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
WDFY3 was identified in a screen for phosphatidylinositol 3 phosphate binding proteins. The FYVE domain is a zinc binding domain found in many PtdIns binding proteins and is proposed to target proteins to membrane lipids. WDFY3 has been shown localized to the nuclear membrane and within autophagic membranes and it is proposed to function as a link between protein aggregates and the autophagic machinery. WDFY3 is also known as autophagy linked FYVE protein. Subcellular Location: Membrane; Peripheral membrane protein; Cytoplasmic side. Note: Colocalizes with autophagic structures in starved cells. Tissue Specificity: Ubiquitous. Similarity: Contains 1 BEACH domain. Contains 1 FYVE-type zinc finger. Contains 5 WD repeats. SWISS: Q8IZQ1 Gene ID: 23001 Database links: Entrez Gene: 23001 Human SwissProt: Q8IZQ1 Human |
| 產品圖片 |
Paraformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (WDFY3) Polyclonal Antibody, Unconjugated (bs-9924R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (WDFY3) Polyclonal Antibody, Unconjugated (bs-9924R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
