| 產品編號 |
bs-0295P-BF488 |
| 英文名稱 |
Rabbit IgG, BF488 conjugated
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| 中文名稱 |
BF488標記兔IgG（同型對照) |
| 別 名 |
Rabbit Immunoglobulin G/BF488 Isotype Control 熒光素Alex熒光素BF488標記兔IgG(同型對照) |
| 性 狀 |
Liquid |
| 保存條件 |
Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 |
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產品介紹 |
Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
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| 產品圖片 |
Blank control: U-937.
Isotype Control: Rabbit IgG-AF488(bs-0295P-BF488)
Dilution: 1ug/Test;
Primary Antibody (green line): Rabbit Anti-AIF1/Iba1 antibody (bs-1363R-AF488)
Dilution: 1ug/Test;
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Isotype control antibody (orange line) was rabbit IgG .Acquisition of 20,000 events was performed.
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