| 產(chǎn)品編號 | bs-1554R |
| 英文名稱 | Rabbit Anti-Aquaporin 5 antibody |
| 中文名稱 | 水通道蛋白5抗體 |
| 別 名 | Aquaporin 5; AQP5; AQP 5; AQP-5; AQP5_HUMAN; Aquaporin-5. |
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Specific References (10) | bs-1554R has been referenced in 10 publications.
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| 研究領(lǐng)域 | 信號轉(zhuǎn)導(dǎo) 通道蛋白 上皮細胞 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat (predicted: Pig,Sheep,Cow,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=1μg/Test,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 29kDa |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse AQP5: 201-265/265 <Cytoplasmic> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Aquaporin 5 (AQP5) is a water channel protein. Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein (MIP or AQP0). Aquaporin 5 plays a role in the generation of saliva, tears and pulmonary secretions. AQP0, AQP2, AQP5, and AQP6 are closely related and all map to 12q13. [provided by RefSeq, Jul 2008] Function: Forms a water-specific channel. Implicated in the generation of saliva, tears, and pulmonary secretions. Subcellular Location: Membrane; Multi-pass membrane protein. Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family. SWISS: Q9WTY4 Gene ID: 11830 Database links: Entrez Gene: 362 Human Entrez Gene: 11830 Mouse Omim: 600442 Human SwissProt: P55064 Human SwissProt: Q9WTY4 Mouse Unigene: 298023 Human Unigene: 45580 Mouse Unigene: 10066 Rat 水通道蛋白(Aquaporin,AQP)是一組與水分子通透有關(guān)的特異性細胞膜轉(zhuǎn)運蛋白。其中,AQP 5在哺乳動物氣管、支氣管的上皮細胞表達較常見. |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Mouse Lung tissue lysates
Lane 2: Rat Stomach tissue lysates
Primary: Anti-Aquaporin 5 (bs-1554R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 29 kD
Observed band size: 27 kD
Paraformaldehyde-fixed, paraffin embedded (rat lung tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Aquaporin 5) Polyclonal Antibody, Unconjugated (bs-1554R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Tissue/cell: human laryngo carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Aquaporin 5 Polyclonal Antibody, Unconjugated(bs-1554R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control: Hela(blue). Primary Antibody:Rabbit Anti-Aquaporin 5 antibody(bs-1554R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min). Antibody (bs-1554R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody of bs-1554R at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.
Blank control: A549(blue). Primary Antibody:Rabbit Anti-Aquaporin 5 antibody(bs-1554R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (bs-1554R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.
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